This is a new course for anyone who want to familiarize themselves with techniques of protein isolation, purification and characterization. protein sample preparation
After completition of this module you will be able to:
- Understand the basic workflow of protein sample preparation
- Use protein sample preparation and purification techniques in a practical setting.
- Develop specific protocols for protein extraction
Following topics will be addressed:
- Protein extraction
- Affinity Chromatography (AC)
- Ion exchange chromatography (IEX)
- Size exclusion chromatography (SEC)
- Cleanup methods (desalting, buffer exchange, dialysis)
Number of course hours : 6 hours
Date : June 2022
Level: Beginners