Protein purificación 2022
This is a new course for anyone who want to familiarize themselves with techniques of protein isolation, purification and characterization. protein sample preparation
After completition of this module you will be able to:
Understand the basic workflow of protein sample preparation
Use protein sample preparation and purification techniques in a practical setting.
Develop specific protocols for protein extraction
Following topics will be addressed:
Protein extraction
Affinity Chromatography (AC)
Ion exchange chromatography (IEX)
Size exclusion chromatography (SEC)
Cleanup methods (desalting, buffer exchange, dialysis)