This course builds on the core skills introduced in the Introduction to R, Introduction to Unix and Introduction to SeqMonk courses to provide a more in depth look at the analysis of bisulfite sequencing data. The course is a mix of theoretical lectures and hands-on practicals which go through the whole analysis pipeline, starting from raw sequence data and covering QC, visualisation, quantitation and differential methylation analysis.
Number of course hours : 8
Level: Expert
Topics Covered:
Course Content:
- The theoretical basis for BS-Seq
- Processing raw sequencing data with Bismark
- Visualisation and exploration of methylation calls with SeqMonk
- The theory of differential methylation calling
- Differential methylation analysis practical