This course gives a practical introduction to the processing, qc and analysis of a simple single cell RNA-Seq experiment performed on the 10X platform. It explains the technology used to create the data and goes through some common analysis tools. The course also goes through the theory and practice of the dimension reduction techniques which are very often used to present this kind of data.
Number of course hours : 8
Level: Beginners
Topics Covered:
Course Content:
- How 10X scRNA libraries are made
- Processing raw data with CellRanger and assessing quality
- Dimension reduction theory - PCA and tSNE
- Reviewing processed data with the Loupe Browser
- R package systems for scRNA analysis
- Using Seurat to analyse 10X data