This course provides an introduction to the methods, data and analysis of quantitative proteomics data. It goes through the background of how the data is acquired and quantitated and the process of searching the spectra against reference databases to identify them at the spectrum, peptide and protein level. We look at quality control of search results to identify problems.
Data analysis is run using the MSstats package, both via the friendly Shiny interface, and then in more detail using R. Whilst there are no strict pre-requisites for this course, a familiatity with R and ggplot would be very helpful.
Number of course hours : 8
Level: Beginners
Topics Covered:
Course Content:
- The theory of proteomics mass spectrometry
- Acquiring RAW data files
- How database searches work
- Analysing data in MSstats