Different targeted proteomics strategies have emerged in the field of proteomics that enable the detection and quantification of subsets of proteins with a high degree of sensitivity and reproducibility across many samples. This EMBO Practical Course
aims to fill in the gap between theory and the actual implementation of the targeted proteomics workflow, so that by the end of the week the students have the necessary know-how to implement different targeted proteomics workflows in their own research
laboratories. The course will offer a daily keynote talk, followed by lectures and practical demonstrations, as well as practical work and exercises that will cover the complete workflow for experimental design and data analysis of targeted proteomics
assays (i.e. targeted method design, optimization of instrument settings, manual and automated quantitative analysis, and statistical analysis of the data). Moreover, the course will host virtual poster sessions and social events to introduce informal
discussion and adapt the technology to each participant's particular interests.
- Introduction to light microscopy and microscope components
- Introduction to Fluorescence Bioimaging
- 3D Fluorescence Imaging of Cells and Tissues
- Quantitative live - cell imaging
- Bioimage data analysis
- Super-resolution microscopy
- Custom-built systems and Core imaging units